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- Title
FIRST REPORT OF COTTON LEAF CURL BUREWALA VIRUS INFECTING RICINUS COMMUNIS.
- Authors
Fareed, M. I.; Tahir, M.; Briddon, R. W.
- Abstract
Castor bean (Ricinus communis; family Euphorbiaceae) is cultivated for the production of oil and as an ornamental plant throughout tropical regions. Leaf samples from castor bean plants with leaf curl and vein thickening were collected from areas around Okara (Pakistan) in 2011. PCR amplification using diagnostic primers showed the presence of a begomovirus and subsequently the specific primer pair (BurNF 5'-CCATGGTTGTGGCAGTTGATTGACAGATAC-3'; BurNR 5'-CCATGGATT CACGCACAGGGGAACCC-3') was used to amplify and clone the whole genome of the virus. The complete nucleotide sequence was determined to be 2,759 nt (accession No. HE985227). Alignments showed the highest levels of nucleotide sequence identity (98.8%) with Cotton leaf curl Burewala virus (CLCuBuV; accession No. JF416947). The virus in castor beans lacks on intact C2 gene, as is typical of CLCuBuV in cotton (Am-rao et al., 2010). An amplification product of ca. 1.4 kb was obtained in PCR with primers for betasatellites (Briddon et al., 2002) and the complete nucleotide sequence of a clone was determined to be 1373 nt (HE985228). The sequence showed 96.3% nucleotide sequence identity to the recombinant Cotton leaf curl Multan betasatellite (CLCuMB; JF502389). This is the first report of CLCuBuV and its betasatellite infecting castor bean, showing this plant species as an alternate host of the virus.
- Subjects
PAKISTAN; CASTOR beans; BEGOMOVIRUSES; VIRUS diseases of plants; PLANT diseases; NUCLEOTIDE sequence; HOST plants; AGRICULTURAL pests
- Publication
Journal of Plant Pathology, 2012, Vol 94, Issue 4, pS4.102
- ISSN
1125-4653
- Publication type
Article