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- Title
A novel allele of monoecious ( m) locus is responsible for elongated fruit shape and perfect flowers in cucumber ( Cucumis sativus L.).
- Authors
Tan, Junyi; Tao, Qianyi; Niu, Huanhuan; Zhang, Zhen; Li, Dandan; Gong, Zhenhui; Weng, Yiqun; Li, Zheng
- Abstract
Key message: A 14 bp deletion in CsACS2 gene encoding a truncated loss-of-function protein is responsible for elongated fruit shape and perfect flowers in cucumber. Abstract: In cucumber ( Cucumis sativus L.), sex expression and fruit shape are important components of biological and marketable yield. The association of fruit shape and sex expression is a very interesting phenomenon. The sex determination is controlled primarily by the F (female) and M (monoecy) loci. Homozygous recessive mm plants bear bisexual (perfect) flowers, and the fruits are often round shaped. CsACS2 encoding the 1-aminocyclopropane-1-carboxylic acid synthase has been shown to be the candidate gene for the m locus. We recently identified an andromonoecious cucumber line H38 that has bisexual flowers but elongated fruits. To rapidly clone this monoecious gene in H38, we developed a tri-parent mapping strategy, which took advantage of the high-density Gy14 × 9930 cucumber genetic map and the powder of bulk segregant analysis. Microsatellite markers from the Gy14 × 9930 map were used to screen two pairs of unisexual and bisexual bulks constructed from H38 × Gy14 and H38 × 9930 F populations. Polymorphic markers were identified and used to quickly develop a framework map and place the monoecious locus of H38 in cucumber chromosome 1. Further fine mapping allowed identification of a novel allele, m- 1, at the monoecious locus to control the bisexual flower in H38, which was due to a 14 bp deletion in the third exon of the CsACS2 gene encoding a truncated loss-of-function protein of the cucumber 1-aminocyclopropane-1-carboxylic acid synthase. This new allele provides a valuable tool in understanding the molecular mechanisms of CsACS2 in the relationships of sex determination, fruit shape, and CsACS activities in cucumber.
- Subjects
CUCUMBER genetics; FRUIT physiology; CARBOXYLIC acids; CHEMICAL synthesis; PLANT gene mapping; GENETIC sex determination
- Publication
Theoretical & Applied Genetics, 2015, Vol 128, Issue 12, p2483
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-015-2603-0