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- Title
Alkaline Ceramidase Mediates the Oxidative Stress Response in Drosophila melanogaster Through Sphingosine.
- Authors
Zhang, Chun-Hong; Zhang, Min-Jing; Shi, Xiao-Xiao; Mao, Cungui; Zhu, Zeng-Rong
- Abstract
Alkaline ceramidase (Dacer) in Drosophila melanogaster was demonstrated to be resistant to paraquat-induced oxidative stress. However, the underlying mechanism for this resistance remained unclear. Here, we showed that sphingosine feeding triggered the accumulation of hydrogen peroxide (H2O2). Dacer -deficient D. melanogaster (Dacer mutant) has higher catalase (CAT) activity and CAT transcription level, leading to higher resistance to oxidative stress induced by paraquat. By performing a quantitative proteomic analysis, we identified 79 differentially expressed proteins in comparing Dacer mutant to wild type. Three oxidoreductases, including two cytochrome P450 (CG3050 , CG9438) and an oxoglutarate/iron-dependent dioxygenase (CG17807), were most significantly upregulated in Dacer mutant. We presumed that altered antioxidative activity in Dacer mutant might be responsible for increased oxidative stress resistance. Our work provides a novel insight into the oxidative antistress response in D. melanogaster.
- Subjects
DIOXYGENASES; DROSOPHILA melanogaster; OXIDATIVE stress; SPHINGOSINE; CYTOCHROME P-450; HYDROGEN peroxide
- Publication
Journal of Insect Science, 2019, Vol 19, Issue 3, pN.PAG
- ISSN
1536-2442
- Publication type
Article
- DOI
10.1093/jisesa/iez042