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- Title
Shoot regeneration from leaf explants of Brunfelsia calycina.
- Authors
Raya Liberman; Liat Shahar; Ada Nissim-Levi; Dalia Evenor; Moshe Reuveni; Michal Oren-Shamir
- Abstract
Abstract  A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23â2.46 μM indole butrytic acid (IBA), they developed roots.
- Subjects
REGENERATION (Botany); PLANT shoots; BRUNFELSIA; EFFECT of temperature on plants; PLANT roots; PLANT development; FOLIAGE plants; PHOTOPERIODISM; PLANTS
- Publication
Plant Cell, Tissue & Organ Culture, 2010, Vol 100, Issue 3, p345
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-009-9642-7