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- Title
Paeoniflorin Attenuates Inflammatory Pain by Inhibiting Microglial Activation and Akt-NF-κB Signaling in the Central Nervous System.
- Authors
Xiaomin Zhang; Bo Hu; Guangtao Xu; Long Xu; Hong Zhou; Zhenyi Ma; Xiaohua Shen; Jia Zhu; Ruilin Shen
- Abstract
Background/Aims: Paeoniflorin (PF) is known to have anti-inflammatory and paregoric effects, but the mechanism underlying its analgesic effect remains unclear. The aim of this study was to clarify the effect of PF on Freund's complete adjuvant (CFA)-induced inflammatory pain and explore the underlying molecular mechanism. Methods: An inflammatory pain model was established by intraplantar injection of CFA in C57BL/6J mice. After intrathecal injection of PF daily for 8 consecutive days, thermal and mechanical withdrawal thresholds, the levels of inflammatory factors TNF-α, IL-1β and IL-6, microglial activity, and the expression of Akt- NF-κB signaling pathway in the spinal cord tissue were detected by animal ethological test, cell culture, enzyme-linked immunosorbent assay, immunofluorescence histochemistry, and western blot. Results: PF inhibited the spinal microglial activation in the CFA-induced pain model. The production of proinflammatory cytokines was decreased in the central nervous system after PF treatment both in vivo and in vitro. PF further displayed a remarkable effect on inhibiting the activation of Akt-NF-κB signaling pathway in vivo and in vitro. Conclusion: These results suggest that PF is a potential therapeutic agent for inflammatory pain and merits further investigation.
- Subjects
CENTRAL nervous system; MICROGLIA; ENZYME-linked immunosorbent assay; IMMUNOFLUORESCENCE; HISTOCHEMISTRY
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2018, Vol 47, Issue 2, p842
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000490076