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- Title
cDNA cloning and characterization of a new stress-responsive gene BoRS1 from Brassica oleracea var. acephala.
- Authors
Dongqin Tang; Hongmei Qian; Shunwu Yua; Youfang Cao; Zhihua Liao; Lingxia Zhao; Xiaofen Sun; Danfeng Huang; Kexuan Tang
- Abstract
A new stress-responsive gene BoRS1 (GenBank accession No.) was isolated from Brassica oleracea var. acephala by rapid amplification of cDNA ends (RACE). The full-length cDNA of BoRS1 was 2076 bp and contained a 1851 bp open reading frame (ORF) encoding 617 amino acids. Sequence analysis indicated that the deduced BoRS1 shared some identities with , RD29A, RD29B and from Arabidopsis thaliana. Southern blot analysis of genomic DNA indicated that other related genes existed and there were two copies of BoRS1 in the genome of B. oleracea. Northern blot analysis revealed that BoRS1 was up-regulated by cold, mannitol, NaCl and abscisic acid (ABA). Expressional fluctuation of time course with ABA implied a two-step induction process. Tissue-specific expression analysis indicated that BoRS1 was expressed in all the tested plant tissues including leaves, stems and roots. Our studies imply that BoRS1 is a new gene that is responsive to environmental stresses such as low temperature, salinity and osmotic stress.
- Subjects
BRASSICA; BIVALVES; GENETIC engineering; ARABIDOPSIS; TISSUES; NUCLEIC acids
- Publication
Physiologia Plantarum, 2004, Vol 121, Issue 4, p578
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.2004.00369.x