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- Title
Gibberellin biosynthesis inhibitor mepiquat chloride enhances root K<sup>+</sup> uptake in cotton by modulating plasma membrane H<sup>+</sup>-ATPase.
- Authors
Zhang, Rui; Wang, Ning; Li, Shuying; Wang, Yiru; Xiao, Shuang; Zhang, Yichi; Eneji, A Egrinya; Zhang, Mingcai; Wang, Baomin; Duan, Liusheng; Li, Fangjun; Tian, Xiaoli; Li, Zhaohu
- Abstract
Potassium deficiency causes severe losses in yield and quality in crops. Mepiquat chloride, a plant growth regulator, can increase K+ uptake in cotton (Gossypium hirsutum), but the underlying physiological mechanisms remain unclear. In this study, we used a non-invasive micro-test technique to measure K+ and H+ fluxes in the root apex with or without inhibitors of K+ channels, K+ transporters, non-selective cation channels, and plasma membrane H+-ATPases. We found that soaking seeds in mepiquat chloride solution increased the K+ influx mediated by K+ channels and reduced the K+ efflux mediated by non-selective cation channels in cotton seedlings. Mepiquat chloride also increased negative membrane potential (E m) and the activity of plasma membrane H+-ATPases in roots, due to higher levels of gene expression and protein accumulation of plasma membrane H+-ATPases as well as phosphorylation of H+-ATPase 11 (GhAHA11). Thus, plasma membrane hyperpolarization mediated by H+-ATPases was able to stimulate the activity of K+ channels in roots treated with mepiquat chloride. In addition, reduced K+ efflux under mepiquat chloride treatment was associated with reduced accumulation of H2O2 in roots. Our results provide important insights into the mechanisms of mepiquat chloride-induced K+ uptake in cotton and hence have the potential to help in improving K nutrition for enhancing cotton production.
- Subjects
CELL membranes; COTTON; PHYSIOLOGY; TRP channels; BIOSYNTHESIS; MEMBRANE potential; PLANT regulators; COTTON growing
- Publication
Journal of Experimental Botany, 2021, Vol 72, Issue 18, p6659
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erab302