We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
NRAS Mutation Is the Sole Recurrent Somatic Mutation in Large Congenital Melanocytic Nevi.
- Authors
Charbel, Christelle; Fontaine, Romain H; Malouf, Gabriel G; Picard, Arnaud; Kadlub, Natacha; El-Murr, Nizar; How-Kit, Alexandre; Su, Xiaoping; Coulomb-L'Hermine, Aurore; Tost, Jorg; Mourah, Samia; Aractingi, Selim; Guégan, Sarah
- Abstract
Congenital melanocytic nevus (CMN) is a particular melanocytic in utero proliferation characterized by an increased risk of melanoma transformation during infancy or adulthood. NRAS and BRAF mutations have consistently been reported in CMN samples, but until recently results have been contradictory. We therefore studied a series of large and giant CMNs and compared them with small and medium CMNs using Sanger sequencing, pyrosequencing, high-resolution melting analysis, and mutation enrichment by an enhanced version of ice-COLD-PCR. Large-giant CMNs displayed NRAS mutations in 94.7% of cases (18/19). At that point, the role of additional mutations in CMN pathogenesis had to be investigated. We therefore performed exome sequencing on five specimens of large-giant nevi. The results showed that NRAS mutation was the sole recurrent somatic event found in such melanocytic proliferations. The genetic profile of small-medium CMNs was significantly different, with 70% of cases bearing NRAS mutations and 30% showing BRAF mutations. These findings strongly suggest that NRAS mutations are sufficient to drive melanocytic benign proliferations in utero.
- Subjects
MELANOMA; ADULTS; SOMATIC mutation; POLYMERASE chain reaction; CARCINOGENESIS; BRENNER tumors
- Publication
Journal of Investigative Dermatology, 2014, Vol 134, Issue 4, p1067
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/jid.2013.429