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- Title
Genotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients.
- Authors
Lourenço, M. C. S.; Grinsztejn, B.; Fandinho-Montes, F. C. O.; Silva, M. G.; Saad, M. H. F.; Fonseca, L. S.; Lourenço, M C; Fandinho-Montes, F C; da Silva, M G; Saad, M H
- Abstract
We investigated whether the recurrence of tuberculosis in HIV-infected patients is due to an exogenous reinfection or relapses after antituberculosis chemotherapy. We reviewed clinical information on 32 patients at a Rio de Janeiro hospital from whom multiple Mycobacterium tuberculosis isolates were taken. All isolates were analysed by DRE-PCR fingerprinting technique, and those with identical DRE-PCR patterns were analysed by the RFLP method. Twenty patients had M. tuberculosis simultaneously isolated from different organs. These patients and nine others with sequential positive cultures after 2 months of therapy showed stable DRE-PCR and RFLP patterns. One patient's isolate became resistant to isoniazid, but the molecular pattern remained unchanged despite the development of drug resistance. In three patients, the DRE-PCR patterns of the isolates changed dramatically. Clinical and microbiological evidence was consistent with active tuberculosis caused by a new strain of M. tuberculosis. The exogenous reinfection of the three patients was not due to an outbreak, but the isolates from each patient showed unique patterns.
- Subjects
BRAZIL; RIO de Janeiro (Brazil); GENETICS of tuberculosis; MYCOBACTERIUM tuberculosis; HIV infection complications; DISEASE relapse
- Publication
Tropical Medicine & International Health, 2000, Vol 5, Issue 7, p488
- ISSN
1360-2276
- Publication type
journal article
- DOI
10.1046/j.1365-3156.2000.00583.x