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- Title
Imaging individual mRNA molecules using multiple singly labeled probes.
- Authors
Raj, Arjun; van den Bogaard, Patrick; Rifkin, Scott A.; van Oudenaarden, Alexander; Tyagi, Sanjay
- Abstract
We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.
- Subjects
MESSENGER RNA; IMAGE analysis; NUCLEIC acid probes; MOLECULAR probes; FLUORESCENCE microscopy; SINGLE cell proteins
- Publication
Nature Methods, 2008, Vol 5, Issue 10, p877
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1253