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- Title
Endogenous and Exogenous Calcium Involved in the Betulin Production from Submerged Culture of Phellinus linteus Induced by Hydrogen Sulfide.
- Authors
Fan, Guizhi; Jian, Duan; Sun, Meiling; Zhan, Yaguang; Sun, Feifei
- Abstract
Using pharmacological and biochemical approaches, Ca involved in the betulin production in mycelia of Phellinus linteus induced by hydrogen sulfide (HS) were investigated. The results showed that 2 mM HS donor NaHS or 10 mM CaCl was found to enhance the betulin content in the mycelia of Phellinus to the maximum, which were 112.43 and 93.24 % higher than that in the control, respectively. Further, NaHS and CaCl co-treatment also showed positive outcome, which were 128.95 or 24.52 % higher than that in the control or NaHS treatment. At the same time, NaHS also enhanced the content of Ca and CaM. But, the above positive inductive effects for Ca, CaM, and betulin production can be blocked with either Ca channel blocker (LaCl, 2-aminoethoxydiphenyl borate) or Ca chelator (ethylenediaminetetraacetic acid (EDTA)). Among of them, betulin content was reduced 35.06 % by NaHS and EGTA to the minimum, and this reduction could be reversed by the application of CaCl (NaHS + EGTA + CaCl). From above results, it can be concluded that endogenous and exogenous calcium involved in the betulin production from submerged culture of P. linteus induced by hydrogen sulfide.
- Subjects
BETULIN; PHELLINUS; HYDROGEN sulfide; ETHYLENEDIAMINETETRAACETIC acid research; CALCIUM ions
- Publication
Applied Biochemistry & Biotechnology, 2016, Vol 178, Issue 3, p594
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-015-1896-4