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- Title
Berberine-induced growth inhibition of epithelial ovarian carcinoma cell lines.
- Authors
Park, Kyoung Sik; Kim, Jong Bin; Lee, Sun Joo; Bae, Jaeman
- Abstract
Aim: The aim of this study is to analyze the impact of berberine on the two human epithelial ovarian carcinoma cell lines OVCAR-3 and SKOV-3 in relation to the potential usefulness of berberine in the treatment of epithelial ovarian cancer. Methods: Under adherent culture conditions, the cell lines were treated with berberine and analyzed for changes in cell growth. The cell cycle duration and degree of apoptosis were evaluated by means of propidium iodide staining and Annexin V staining. Results: After the berberine treatment, the two cell lines showed a dose-dependent reduction in the growth rate. In the cell cycle analysis, the OVCAR-3 and SKOV-3 cells showed an increased DNA content of 5% in the G2/M phase and 7% in the S phase, respectively. Additionally, the results confirm the cell cycle arrest by immunoblotting and the up-regulation of p27; however, in the apoptosis analysis, neither cell line showed an increase in apoptosis after the berberine treatment. Conclusion: Berberine treatment can inhibit proliferation through a cell cycle arrest in OVCAR-3 and SKOV-3 cells. Thus, berberine may be a novel anticancer drug for the treatment of ovarian cancer.
- Subjects
THERAPEUTIC use of alkaloids; THERAPEUTIC use of plant extracts; APOPTOSIS; RESEARCH methodology; OVARIAN tumors; RESEARCH funding; T-test (Statistics); TISSUE culture; DESCRIPTIVE statistics
- Publication
Journal of Obstetrics & Gynaecology Research, 2012, Vol 38, Issue 3, p535
- ISSN
1341-8076
- Publication type
Article
- DOI
10.1111/j.1447-0756.2011.01743.x