We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The secretome obtained under hypoxic preconditioning from human adipose-derived stem cells exerts promoted anti-apoptotic potentials through upregulated autophagic process.
- Authors
Seo, Haeyeon; Choi, Ho Joong; Kim, Ok-Hee; Park, Jung Hyun; Hong, Ha Eun; Kim, Say-June
- Abstract
Background: Hypoxic preconditioning (HP) is a stem cell preconditioning modality designed to augment the therapeutic effects of mesenchymal stem cells (MSCs). Although autophagy is expected to play a role in HP, very little is known regarding the relationship between HP and autophagy. Methods and results: The adipose-derived stem cell (ASC)-secretome obtained under normoxia (NCM) and ASC-secretome obtained under HP (HCM) were obtained by culturing ASCs for 24 h under normoxic (21% partial pressure of O2) and hypoxic (1% partial pressure of O2) conditions, respectively. Subsequently, to determine the in vivo effects of HCM, each secretome was injected into 70% partially hepatectomized mice, and liver specimens were obtained. HCM significantly reduced the apoptosis of thioacetamide-treated AML12 hepatocytes and promoted the autophagic processes of the cells (P < 0.05). Autophagy blockage by either bafilomycin A1 or ATG5 siRNA significantly abrogated the anti-apoptotic effect of HCM (P < 0.05), demonstrating that HCM exerts its anti-apoptotic effect by promoting autophagy. The effect of HCM — reduction of cell apoptosis and promotion of autophagic process — was also demonstrated in a mouse model. Conclusions: HP appears to induce ASCs to release a secretome with enhanced anti-apoptotic effects by promoting the autophagic process of ASCs.
- Subjects
HUMAN stem cells; MESENCHYMAL stem cells; STEM cells; PARTIAL pressure; LIVER cells
- Publication
Molecular Biology Reports, 2022, Vol 49, Issue 9, p8859
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-022-07736-z