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- Title
Intravenous immunoglobulin is effective in alleviating hepatic ischemia–reperfusion injury: a rat model study.
- Authors
Chen, Zeming; Lin, Runzhui; Zhuo, Hua; Xu, Fengjie; Liu, Xingmu
- Abstract
Background: Hepatic ischemia–reperfusion injury (I/R) is an important factor affecting the prognosis of patients undergoing liver surgery. This study aimed to explore the value of intravenous immunoglobulin (IVIG) in hepatic I/R and its mechanism in a rat model. Materials and methods: Forty eight adult male Sprague–Dawley (SD) rats were divided into six groups randomly: (1–2) treated with normal saline (NS) without ischemia or reperfusion; (3–4) treated with NS + 30 min ischemia; (5–6) treated with IVIG + 30 min ischemia. Rats of group 1/3/5 were euthanized at 12 h after operation (sham + NS + 12 h, I/R + NS + 12 h, I/R + IVIG + 12 h group) while group 2/4/6 were euthanized at 24 h (sham + NS + 24 h, I/R + NS + 24 h, I/R + IVIG + 24 h group). Interleukin 10 (IL-10), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) were quantified as well as serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Hepatic pathological changes were observed while nuclear factor kappa B p65 (NF-κB p65), Inhibitory Subunit of NF Kappa B Alpha (IKB-alpha) and cleaved caspase-3 were detected. Conclusion: ALT, AST, IL-6, TNF-alpha, NF-κB p65 and cleaved caspase-3 were increased by I/R whereas IL-10 and IKB-alpha were decreased. However, IVIG pretreatment reduced ALT, AST, IL-6, TNF-alpha, NF-κB p65 and cleaved caspase-3, but increased IL-10 and IKB-alpha. IVIG treatment attenuates the infiltration of inflammatory cell and cell apoptosis which were observed in I/R groups. IVIG may alleviate hepatic I/R in rats by inhibiting the classical NF-κB signaling pathway, reducing IL-6, TNF-alpha, promoting IL-10, and inhibiting cell apoptosis.
- Subjects
ASPARTATE aminotransferase; REPERFUSION injury; TUMOR necrosis factors; NF-kappa B; ANIMAL disease models; PATHOLOGICAL physiology
- Publication
Molecular Biology Reports, 2022, Vol 49, Issue 1, p341
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-021-06879-9