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- Title
Cloning of Genes Differentially Expressed during the Initial Stage of Fruit Development in Melon (Cucumis melo cv. Reticulatus).
- Authors
Jung Woo Choi; Gun Bo Kim; Yun Chan Huh; Mi Ra Kwon; II Gin Mok; Jung Wan Kim; Tae Soo Lee; Shinje Kim; Kyung Hoan Im
- Abstract
We have cloned genes involved in the initial stage of fruit development in the melon by suppression subtrac-tive hybridization. A cDNA library of unfertilized ovules was subtracted from that of fruit 9 days after pollination (DAP); 10 of the 40 selected cDNA clones were identified by reverse Northern analysis as genes differentially expressed in fruit at 9 DAP. Seven of the ten genes were homologous to genes of known function; two were related to genes with unknown functions, and one was novel. With the exception of cucumisin, none of the cDNAs had been previously identified in melon. According to Northern analyses, six of the genes were expressed at high levels early in fruit development. Expression of cucumisin, Cmf-25, Cmf-30, and Cmf-124 was highest at 9 DAP, implying that these genes are involved in the initial stage of fruit development. Cmf-30, a seed nucellus-specific gene, was also expressed early in seed development. The other genes were expressed at a moderate level throughout fruit development, with the highest expression occurring in fruit at 9 and 18 DAP. In conclusion, nine new genes involved in early fruit development in melon were cloned, and their temporal and spatial expression patterns indicate that they are preferentially expressed during the active growing stage of fruit.
- Publication
Molecules & Cells (Springer Nature), 2004, Vol 17, Issue 2, p237
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13032-9