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- Title
Effects of the Taste Substances and Metal Cations in Green Tea Infusion on the Turbidity of EGCG–Mucin Mixtures.
- Authors
Xu, Longjie; Ye, Qingqing; Cao, Qingqing; Liu, Yuyi; Li, Xinghui; Liu, Zhengquan; Gong, Yushun; Zhang, Sheng; Yin, Junfeng; Xu, Yongquan
- Abstract
Astringency has an important impact on the taste quality of tea infusion, a process which occurs when polyphenols complex with salivary proteins to form an impermeable membrane. (-)-Epigallocatechin gallate (EGCG) is the main astringent compound found in green tea and mucin is the main protein present in saliva. Determining the turbidity of EGCG–mucin mixtures is an effective method to quantify the astringency intensity of EGCG solutions. In this study, the effects of taste-related, substances present during green tea infusion, on the turbidity of EGCG–mucin mixtures was investigated under the reacting conditions of a pH value of 5.0, at 37 °C, and for 30 min. The results showed that epicatechins, caffeic acid, chlorogenic acid, and gallic acid reduced the turbidity of EGCG–mucin mixtures, while rutin increased turbidity. Metal ions increased the turbidity of EGCG–mucin mixtures. These can be arranged by effectiveness as Al3+ > K+ > Mg2+ > Ca2+. Caffeine, theanine, and sodium glutamate all decreased the turbidity values of EGCG–mucin mixtures, but sucrose had a weak effect. Further experiments confirmed that the turbidity of green tea infusion–mucin mixture indicated the astringent intensity of green tea infusion, and that the turbidity was significantly correlated with the contents of tea polyphenols and EGCG.
- Subjects
EPIGALLOCATECHIN gallate; GREEN tea; TURBIDITY; MONOSODIUM glutamate; SALIVARY proteins; CHLOROGENIC acid; GALLIC acid
- Publication
Foods, 2024, Vol 13, Issue 8, p1172
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods13081172