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- Title
Mesoporous TiO<sub>2</sub> nanoparticles: A new material for biolistic bombardment.
- Authors
Hou, Liyuan; Wang, Pengyu; Kong, Fantao; Park, Hyunsun; Kobiro, Kazuya; Ohama, Takeshi
- Abstract
To date, only solid heavy metals such as gold or tungsten have been used as DNA carriers in biolistic bombardment of algae. In this study, we show that even a metal oxide of lower density can act as a DNA carrier. We investigated the potency of size-controlled mesoporous titanium dioxide ( TiO2) particles. Among the six tested gas pressures, TiO2 particles best facilitated transformation of the green alga Chlamydomonas reinhardtii at 1100 psi (approximately 7.6 MPa) and 2000 psi (approximately 14 MPa). Surprisingly, a mesoporous metal oxide with a density of approximately only one-tenth that of gold or tungsten could be effective as a DNA carrier in biolistic bombardment of a rigid cell wall-containing alga. In addition, we found two peaks of gas pressures in the transformation ratio irrespective of whether the particles were made of gold, tungsten, or TiO2.
- Subjects
TITANIUM dioxide; NANOPARTICLES; CHLAMYDOMONAS reinhardtii; HEAVY metals; METALLIC oxides; DNA; ALGAE
- Publication
Phycological Research, 2013, Vol 61, Issue 1, p58
- ISSN
1322-0829
- Publication type
Article
- DOI
10.1111/j.1440-1835.2012.00671.x