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- Title
Viral Interferon Regulatory Factor 1 of Kaposi’s Sarcoma-Associated Herpesvirus Interacts with a Translocation Liposarcoma Protein-Associated Serine-Arginine Protein
- Authors
Kim, Sunmi; Jong, Jae Eun; Seo, Taegun
- Abstract
Abstract: Objectives: To confirm that Kaposi’s sarcoma-associated herpes virus open-reading frame K9, viral interferon regulatory factor 1 (vIRF1), interacts with splicing factor, translocation liposarcoma protein-associated serine-arginine protein (TASR), in vivo and to establish whether interactions between vIRF1 and TASRs influence alternative splicing. Methods: Association between vIRF1 and TASRs was confirmed with the glutathione S-transferase pull-down assay and coimmunoprecipitation. Further colocalization was shown by immunofluorescence. The in vivo splicing assay was performed to confirm the alterations in the splicing pattern. Results: vIRF1 interacts with both TASR1 and 2 in vivo. vIRF1 has been shown to colocalize with TASR proteins in 293 T cells. However, an in vivo splicing revealed no alterations in the splicing pattern via interaction. Conclusions: The study data suggest that vIRF1 interacts with the TASR protein. However, vIRF1 interactions do not affect TASR-mediated alternative splicing.
- Subjects
INTERFERON regulatory factors; GLUTATHIONE transferase; KAPOSI'S sarcoma; HERPESVIRUSES; LIPOSARCOMA; T cells; RNA splicing; IMMUNOFLUORESCENCE
- Publication
Osong Public Health & Research Perspectives, 2012, Vol 3, Issue 1, p8
- ISSN
2210-9099
- Publication type
Article
- DOI
10.1016/j.phrp.2012.01.001