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- Title
Development of a tier 1 R5 clade C simian-human immunodeficiency virus as a tool to test neutralizing antibody-based immunoprophylaxis.
- Authors
Siddappa, Nagadenahalli B.; Hemashettar, Girish; Wong, Yin Ling; Lakhashe, Samir; Rasmussen, Robert A.; Watkins, Jennifer D.; Novembre, Francis J.; Villinger, François; Else, James G.; Montefiori, David C.; Ruprecht, Ruth M.
- Abstract
While some recently transmitted HIV clade C (HIV-C) strains exhibited tier 1 neutralization phenotypes, most were tier 2 strains ( J Virol 2010; 84:1439). Because induction of neutralizing antibodies (nAbs) through vaccination against tier 2 viruses has proven difficult, we have generated a tier 1, clade C simian-human immunodeficiency virus (SHIV-C) to permit efficacy testing of candidate AIDS vaccines against tier 1 viruses. SHIV-1157ipEL was created by swapping env of a late-stage virus with that of a tier 1, early form. After adaptation to rhesus macaques (RM), passaged SHIV-1157ipEL-p replicated vigorously in vitro and in vivo while maintaining R5 tropism. The virus was reproducibly transmissible intrarectally. Phylogenetically, SHIV-1157ipEL-p Env clustered with HIV-C sequences. All RM chronically infected with SHIV-1157ipEL-p developed high nAb titers against autologous as well as heterologous tier 1 strains. SHIV-1157ipEL-p was reproducibly transmitted in RM, induced cross-clade nAbs, and represents a tool to evaluate anti-HIV-C nAb responses in primates.
- Subjects
HIV; IMMUNOGLOBULINS; PHENOTYPES; RHESUS monkeys; TROPISMS; PRIMATES; PHYLOGENY; AIDS vaccines; DRUG development
- Publication
Journal of Medical Primatology, 2011, Vol 40, Issue 2, p120
- ISSN
0047-2565
- Publication type
Article
- DOI
10.1111/j.1600-0684.2010.00454.x