We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Cell Cycle-Related FAM64A Could be Activated by TGF-β Signaling to Promote Glioma Progression.
- Authors
Fu, Minjie; Zhang, Jingwen; Zhang, Licheng; Feng, Yuan; Fang, Xinqi; Zhang, Jinsen; Wen, Wenyu; Hua, Wei; Mao, Ying
- Abstract
Gliomas are aggressive brain tumors characterized by uncontrolled cell proliferation. FAM64A, a cell cycle-related gene, has been found to promote cell proliferation in various tumors, including gliomas. However, the regulatory mechanism and clinical significance of FAM64A in gliomas remain unclear. In this study, we investigated FAM64A expression in gliomas with different grades and constructed FAM64A silenced cell lines to study its functions. Our results demonstrated that FAM64A was highly expressed in glioblastoma (P < 0.001) and associated with a poor prognosis (P < 0.001). Expression profiles at the single-cell resolution indicated FAM64A could play a role in a cell-cycle-dependent way to promote glioma cell proliferation. We further observed that FAM64A silencing in glioma cells resulted in disrupted proliferation and migration ability, and increased cell accumulation in the G2/M phase (P = 0.034). Additionally, TGF-β signaling upregulates FAM64A expression, and SMAD4 and FAM64A co-localize in high-grade glioma tissues. We found FAM64A knockdown inhibited TGF-β-induced epithelial-mesenchymal transition in glioma. Our findings suggest that FAM64A could serve as a diagnostic and therapeutic target in gliomas.
- Subjects
GLIOMAS; BRAIN tumors; EPITHELIAL-mesenchymal transition; GLIOBLASTOMA multiforme; CELL proliferation
- Publication
Cellular & Molecular Neurobiology, 2023, Vol 43, Issue 6, p2975
- ISSN
0272-4340
- Publication type
Article
- DOI
10.1007/s10571-023-01348-2