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- Title
In Vitro Characterization and In Vivo Effectiveness of Ebola Virus Specific Equine Polyclonal F(ab')2.
- Authors
Racine, Trina; Denizot, Mélanie; Pannetier, Delphine; Nguyen, Ludovic; Pasquier, Anaïs; Raoul, Hervé; Saluzzo, Jean-François; Kobinger, Gary; Veas, Francisco; Herbreteau, Cécile H
- Abstract
There is no vaccine or approved therapy against lethal Ebola virus (EBOV). We investigated a proven technology platform to produce polyclonal IgG fragments, F(ab')2, against EBOV. Horses immunized with nanoparticles harboring surface glycoprotein trimers of EBOV-Zaire/Makona produced anti-Ebola IgG polyclonal antibodies with high neutralization activity. Highly purified equine anti-Ebola F(ab')2 showed strong cross-neutralization of 2 Zaire EBOV strains (Gabon 2001 and Makona) and in vivo 3 or 5 daily F(ab')2 intraperitoneal injections provided 100% protection to BALB/c mice against lethal EBOV challenge. Rapid preparation of purified equine anti-Ebola F(ab')2 offers a potentially efficient therapeutic approach against EBOV disease in humans.
- Subjects
ANIMAL experimentation; COMPARATIVE studies; EBOLA virus disease; HORSES; IMMUNIZATION; IMMUNOGLOBULINS; RESEARCH methodology; MEDICAL cooperation; MICE; RESEARCH; VIRAL antibodies; EVALUATION research; EBOLA virus
- Publication
Journal of Infectious Diseases, 2019, Vol 220, Issue 1, p41
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiz068