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- Title
Functional Comparison of T Cells Recognizing Cytomegalovirus pp65 and Intermediate-Early Antigen Polypeptides in Hematopoietic Stem-Cell Transplant and Solid Organ Transplant Recipients.
- Authors
Lacey, Simon F.; La Rosa, Corinna; Zhou, Wendy; Sharma, Madeva C.; Martinez, Joy; Krishnan, Aparna; Gallez-Hawkins, Ghislaine; Lia Thao; Longmate, Jeff; Spielberger, Ricardo; Forman, Stephen J.; Limaye, Ajit; Zaia, John A.; Diamond, Don J.
- Abstract
The functional status of cytotoxic T lymphocyte (CTL) populations recognizing cytomegalovirus intermediate-early antigen (IE1) and pp65 polypeptides was investigated in peripheral blood mononuclear cells from hematopoietic stem-cell transplant (HSCT) and solid organ transplant recipients. Combined flow-based CD107a/b degranulation/mobilization and intracellular cytokine (ICC) assays using peptide libraries as antigens indicated that a significantly higher proportion of pp65-specific CTLs were in a more mature functional state, compared with IE1-specific CTLs. Degranulation/multiple cytokine ICC assays also indicated that a significantly higher proportion of pp65-specific than IE1-specific CTLs secreted both interferon-g and tumor necrosis factor- a and possessed greater cytotoxic potential. These results support our earlier findings of functional differences between CTLs recognizing individual epitopes within the IE1 and pp65 antigens in healthy donors and HSCT recipients and extend them to a broader array of human leukocyte antigen-restricted responses to those antigens. We also provide evidence of a relationship between cytotoxic function and the ability of cytomegalovirus-specific CTLs to secrete multiple cytokines.
- Subjects
T cells; VIRUS identification; CYTOMEGALOVIRUSES; STEM cell transplantation; EPITOPES; TUMOR necrosis factors; HLA histocompatibility antigens; COMPLICATIONS from organ transplantation
- Publication
Journal of Infectious Diseases, 2006, Vol 194, Issue 10, p1410
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/508495