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- Title
Is a positive Christie-Atkinson-Munch-Peterson (CAMP) test sensitive enough for the identification of Streptococcus agalactiae?
- Authors
Guo, Dacheng; Xi, Yu; Wang, Shanmei; Wang, Zeyu
- Abstract
<bold>Background: </bold>For a long time, the Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of Streptococcus agalactiae, and a positive result for S.agalactiae has been considered sensitive enough.<bold>Methods: </bold>To confirm whether a positive CAMP test is a requirement for the identification of S.agalactiae, five suspected CAMP-negative S.agalactiae isolates from two hospitals, confirmed as Gram-positive and catalase-negative streptococci, were verified by the CAMP test in three batches of plates from two manufacturers and identified by the Phoenix system, MALDI-TOF MS, the PCR assay and the 16S rDNA gene sequencing.<bold>Results: </bold>All five suspected strains were S.agalactiae, four of which were CAMP-negative and one of which was not S.agalactiae by the PCR assay.<bold>Conclusions: </bold>A positive CAMP test was lacking sensitivity for the identification of S.agalactiae, and the question of whether the cfb gene is worthy of targeting should be further studied.
- Subjects
STREPTOCOCCUS agalactiae; STREPTOCOCCUS; STREPTOCOCCACEAE; GRAM-positive bacteria; RECOMBINANT DNA; STREPTOCOCCAL disease diagnosis; BACTERIOLOGY technique; DNA; MASS spectrometry; POLYMERASE chain reaction; STREPTOCOCCAL diseases; VAGINA
- Publication
BMC Infectious Diseases, 2019, Vol 19, Issue 1, pN.PAG
- ISSN
1471-2334
- Publication type
journal article
- DOI
10.1186/s12879-018-3561-3