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- Title
超高效液相色谱-静电场轨道阱高分辨质谱法测定 水产品中地西泮及丁香酚类化合物残留.
- Authors
殷雪琰; 王洁琼; 刘 笑; 王雨晴; 吴冬梅; 翟云忠
- Abstract
Objective To establish an online purification method for the determination of 4 kinds of diazepam and their metabolites and 6 kinds of eugenol compounds from aquatic products based on Turboflow combined with ultra performance liquid chromatography-electrostatic field orbital high resolution mass spectrometry (UPLC-Q Exactive MS). Methods The samples were purified by on-line Turboflow. The analytes were separated on a AccucoreTM aQ C18 column (100 mm×2.1 mm, 2.6 μm) by gradient elution with water-acetonitrile solution as the mobile phases. The MS analysis was performed by full scan and automatic trigger secondary mass spectrometry monitoring in the positive ion mode. The external standard method was used for quantification. Results Diazepam, its metabolites and eugenol compounds had good linearity in the range of 1.0–100.0 μg/L, and the correlation coefficient (r ² ) of the compounds was greater than 0.995. The limits of quantification in aquatic products were 0.5 μg/kg, and 2.0 μg/kg, respectively. The recoveries were 75.3%–110.0%, and the relative standard deviations were less than 10%. Conclusion The method has the advantages of simple operation, high sensitivity and low detection limit, and is suitable for the determination of diazepam and eugenol residues in aquatic products. Through the investigation, it is found that diazepam and eugenol compounds are detected in aquatic products during transportation, and it is urgent to carry out risk monitoring on the residues of sedatives or anesthetics in aquatic products and strengthen supervision to ensure the quality and safety of aquatic products.
- Publication
Journal of Food Safety & Quality, 2024, Vol 15, Issue 16, p195
- ISSN
2095-0381
- Publication type
Article
- DOI
10.19812/j.cnki.jfsq11-5956/ts.20240607003