We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Enhancing canine semen quality through a second centrifugation after 48 hours of storage: a comparative study.
- Authors
Sinagra, Letizia; Polisca, Angela; Donato, Giulia; Caspanello, Tiziana; Pettina, Giorgia; Pastore, Sara; De Majo, Massimo; Cristarella, Santo; Quartuccio, Marco; Zappone, Viola
- Abstract
Background: Centrifugation is a common procedure to improve the quality of chilled and frozen canine semen by removing debris and seminal plasma and adding semen extenders. The aim of this study was to evaluate the efficacy and influence of a second centrifugation after 48 h of storage at 5 °C on the sperm quality of canine semen. The ejaculates of 45 healthy male dogs, divided into three groups according to body weight, were analyzed for macro- and microparameters such as ejaculate volume, sperm concentration, kinematic parameters, morphology, and integrity of plasma membrane. Samples were analyzed at baseline conditions (T0), after 24 h (T24) and after 48 h (T48) to assess the effects of the different treatments on sperm quality. Results: The results showed a significant effect of a second centrifugation on the improvement of chilled sperm quality compared to the other techniques, especially up to 48 h. Conclusions: Analysis of the data showed that the semen samples centrifuged and then cooled at 5 °C had acceptable semen parameters, especially in terms of motility, with a gradual decrease in serial evaluations after 24 and 48 h. A second centrifugation after 48 h of storage may lead to better semen quality and improve the kinetics of sperm parameters, the percentage of morphologically normal sperm and the percentage of sperm with intact membranes.
- Subjects
SEMEN analysis; SPERM motility; ARTIFICIAL insemination; SEMEN; CELL membranes; FROZEN semen; SPERMATOZOA
- Publication
Acta Veterinaria Scandinavica, 2024, Vol 66, Issue 1, p1
- ISSN
0044-605X
- Publication type
Article
- DOI
10.1186/s13028-024-00767-5