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- Title
Evaluation of placental bed uterine in L-NAMEinduced early-onset preeclampsia (EO-PE) like the rat model.
- Authors
Fitriana, Fitriana; Soetrisno, Soetrisno; Sulistyowati, Sri; Indarto, Dono
- Abstract
Objective: Preeclampsia (PE) is the leading cause of maternal death worldwide and is associated with long-term morbidity in both mothers and newborns. Animal modeling is considered a functional source for understanding PE pathogenesis, diagnostic standards, and therapeutic approaches. Materials and Methods: This study aimed to demonstrate and evaluate the use of N-nitro-L-arginine methyl ester (L-NAME) in a Wistar rat model under conditions similar to PE. A total of 12 rats were divided into 4 groups, each consisting of 3 members, including the pregnant control group and treatment groups administered low-dose (PE 25 mg/kg L-NAME/day), medium-dose (PE 50 mg/kg L-NAME/day), and high-dose L-NAME (PE 75 mg/kg L-NAME/ day) L-NAME from gestational day 4 to 19. Measurements included blood pressure, creatinine, and proteinuria levels, placental histological changes, and placental tissue hypoxia-inducible factor 1-alpha, and plasma endothelial nitric oxide synthase levels. Results: The results showed that intervention with L-NAME at 75 mg/kg body weight/day (PE3) induced PE earlier than that with 50 mg/kg body weight/ day L-NAME. Conclusion: The model conditions also support further research into PE pathogenesis.
- Subjects
PLACENTA; RESEARCH funding; DATA analysis; DESCRIPTIVE statistics; RATS; DISEASES; CONTROL groups; PRE-tests &; post-tests; PREECLAMPSIA; ANIMAL experimentation; ONE-way analysis of variance; STATISTICS; NITRIC-oxide synthases; MICROSCOPY; CONFIDENCE intervals; DATA analysis software; UTERUS
- Publication
Turkish Journal of Obstetrics & Gynecology, 2024, Vol 21, Issue 3, p180
- ISSN
2149-9322
- Publication type
Article
- DOI
10.4274/tjod.galenos.2024.99132