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- Title
Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing.
- Authors
Banos-Mateos, Soledad; Lopez-Robles, Carlos; Yubero, María Eugenia; Jurado, Aroa; Arbelaiz-Sarasola, Ane; Lamsfus-Calle, Andrés; Arrasate, Ane; Albo, Carmen; Ramírez, Juan Carlos; Fertin, Marie J.
- Abstract
Transduction of producer cells during lentiviral vector (LVV) production causes the loss of 70–90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the LDL receptor located on the producer cell membrane, allowing lentiviral vector transduction. Avoiding retro-transduction in LVV manufacturing is crucial to improve net production and, therefore, the efficiency of the production process. Here, we describe a method for quantifying the transduction of producer cells and three different strategies that, focused on the interaction between VSV-G and the LDLR, aim to reduce retro-transduction.
- Subjects
LOW density lipoprotein receptors; PRODUCTION losses; GENE therapy; GENETIC transduction; MANUFACTURING processes
- Publication
Viruses (1999-4915), 2024, Vol 16, Issue 8, p1216
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v16081216