We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Antibiofilm Efficacy of Calcium Silicate-Based Endodontic Sealers.
- Authors
Ruiz-Linares, Matilde; Fedoseev, Vsevolod; Solana, Carmen; Muñoz-Sandoval, Cecilia; Ferrer-Luque, Carmen María
- Abstract
Background: Using endodontic sealers with long-term antimicrobial properties can increase the success of endodontic treatment. This study aimed to assess the antimicrobial activity over time of two calcium silicate (CS)-based sealers, AH Plus Bioceramic and BioRoot RCS, and to compare them with an epoxy resin-based sealer, AH Plus Jet, against mature polymicrobial biofilms grown on human radicular dentin. Methods: The antimicrobial activity of the sealers was tested using a direct contact test after 1 and 6 weeks of contact with the biofilms. Cell viability was determined by the adenosine triphosphate (ATP) method and flow cytometry (FC). The results of the ATP test were analyzed using an ANOVA with Welch's correction, followed by the Games–Howell test. The number of cells with damaged membranes obtained by FC in each period was compared by means of an ANOVA and Duncan's test. For the comparison between times, a Student's t-test was used. Results: Globally, after a week of contact, the epoxy resin-based sealer obtained the best results. However, at 6 weeks, the two CSs showed the highest antimicrobial efficacy, with a significant increase in this activity over time. Conclusions: Calcium silicate-based sealers exert long-term antimicrobial activity against endodontic biofilms.
- Subjects
PIT &; fissure sealants (Dentistry); ADENOSINE triphosphate; CALCIUM silicates; ANTI-infective agents; BIOFILMS
- Publication
Materials (1996-1944), 2024, Vol 17, Issue 16, p3937
- ISSN
1996-1944
- Publication type
Article
- DOI
10.3390/ma17163937