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- Title
Characterization and discrimination of pollen by Raman microscopy.
- Authors
Ivleva, N. P.; Niessner, R.; Panne, U.
- Abstract
The chemical characterization and discrimination of allergy-relevant pollen (common ragweed (Ambrosia artemisiifolia), white birch (Betula pendula), English oak (Quercus robur), and European linden (Tilia cordata)) has been studied by Raman microscopy. Spectra were obtained at different excitation wavelengths (514, 633, and 780 nm) and various methods were examined to minimize the strong fluorescence background. The use of a He-Ne laser (633 nm) for excitation yields high-quality single pollen Raman spectra, which contain multiple bands due to pollen components such as carotenoids, proteins, nucleic acids, carbohydrates, and lipids. Multivariate classification, i.e. principal component analysis (PCA) and hierarchical cluster analysis, demonstrated the validity of the approach for discrimination between different pollen species.
- Subjects
ANALYTICAL chemistry; PALYNOLOGY; LINDENS; ENGLISH oak; RAMAN spectroscopy; MICROSCOPICAL technique
- Publication
Analytical & Bioanalytical Chemistry, 2005, Vol 381, Issue 1, p261
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-004-2942-1