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- Title
Direct Cytosolic Delivery of siRNA Using Nanoparticle-Stabilized Nanocapsules.
- Authors
Jiang, Ying; Tang, Rui; Duncan, Bradley; Jiang, Ziwen; Yan, Bo; Mout, Rubul; Rotello, Vincent M.
- Abstract
The use of nanoparticle-stabilized nanocapsules (NPSCs) for the direct cytosolic delivery of siRNA is reported. In this approach, siRNA is complexed with cationic arginine-functionalized gold nanoparticles by electrostatic interactions, with the resulting ensemble self-assembled onto the surface of fatty acid nanodroplets to form a NPSC/siRNA nanocomplex. The complex rapidly delivers siRNA into the cytosol through membrane fusion, a mechanism supported by cellular uptake studies. Using destabilized green fluorescent protein (deGFP) as a target, 90 % knockdown was observed in HEK293 cells. Moreover, the delivery of siRNA targeting polo-like kinase 1 (siPLK1) efficiently silenced PLK1 expression in cancer cells with concomitant cytotoxicity.
- Subjects
NANOCAPSULES; NANOBIOTECHNOLOGY; NANOPARTICLES; DRUG carriers; BIOTECHNOLOGY
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 2, p516
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201409161