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- Title
Targetable Phosphorescent Oxygen Nanosensors for the Assessment of Tumor Mitochondrial Dysfunction By Monitoring the Respiratory Activity.
- Authors
Xiao-Hui Wang; Hong-Shang Peng; Lin Yang; Fang-Tian You; Feng Teng; Ling-Ling Hou; Wolfbeis, Otto S.
- Abstract
Cellular respiration is a worthwhile criterion to evaluate mitochondrial dysfunction by measuring the dissolved oxygen. However, most of the existing sensing strategies merely report extracellular (ec-) or intracellular (ic-) O2 rather than intramitochondrial (im-) O2. Herein we present a method to assess tumor mitochondrial dysfunction with three phosphorescent nanosensors, which respond to ec-, ic-, and im-O2. Time-resolved luminescence is applied to determine the respective oxygen consumption rates (OCRs) under varying respiratory conditions. Data obtained for the OCRs and on (intra)cellular O2 gradients demonstrate that mitochondria in tumor cells are distinctly less active than those of healthy cells, resulting from restrained glucose utilization of and physical injury to the mitochondria. We believe that such a site-resolved sensing strategy can be applied to numerous other situations, for example to evaluate the adverse effects of drug candidates.
- Subjects
OXYGEN analysis; CELL respiration; NANOPARTICLE synthesis; PHOSPHORS; CHEMICAL synthesis; NANOSENSORS; MITOCHONDRIAL pathology
- Publication
Angewandte Chemie, 2014, Vol 126, Issue 46, p12679
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201405048