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- Title
Revisiting the effect of water content on enzymatic peptide synthesis in non-aqueous medium.
- Authors
Shin, Jong-Shik; Kim, Byung-Gee
- Abstract
Enzymatic acyl-transfer reaction in organic medium competes with the hydrolytic side reaction depending on the water content. The effect of water content on aminolysis activity of α-chymotrypsin for the synthesis of Bz-Tyr-Val-NH2 in acetonitrile was examined under the conditions which were devoid of the hydrolytic deacylation. Excess H-Val-NH2 (880 mM) was employed to keep the hydrolysis negligible. The aminolysis rate increased abruptly between 4 and 5% (v/v) water but a further increase in the water content did not affect the reaction rate. This suggests that water added more than 5% (v/v) does not enhance intrinsic enzyme activity but acts only as a nucleophile for the hydrolytic deacylation.
- Subjects
DIGESTIVE enzymes; ENZYMES; CHYMOTRYPSIN; SERINE proteinases; HYDROLYSIS; CHEMICAL kinetics
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 22, p1903
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1020952109068