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- Title
Extracellular K5 polysaccharidic antigen recovery performed by different cell separation technologies.
- Authors
Manzoni, Matilde; Bergomi, Silvia; Rollini, Manuela; Cavazzoni, Valeria; Felisaz, Christophe; Marinoni, Carlo
- Abstract
The K5 polysaccharidic antigen obtainable from a strain of Escherichia coli is the non-sulphated precursor in heparin biosynthesis; K5 is composed by two components, 16 000 and 1500 Da, their ratio depending on the fermentation conditions. In this study an assessment was made of how various cell-culture filtrate separation technologies affected the components themselves and their ratio. A dynamic membrane filtration technology, a tubular ceramic module for tangential flow microfiltration and the standard discontinuous centrifugation were comparatively employed to perform the separation. Experiments carried out on E. coli cultures containing the two components in different ratios showed that the DMF system is a suitable technology for K5 isolation.
- Subjects
ESCHERICHIA coli; BIOCHEMICAL engineering; INDUSTRIAL microbiology; ANTIGENS; FERMENTATION; CELL culture
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 9, p759
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005612405051