We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Novel cap-independent translation with the 5′-noncoding region of L-A virus mRNA.
- Authors
Ebihara, Takashi; Yanagida, Yasuko; Haruyama, Tetsuya; Kobatake, Eiry; Aizawa, Masuo
- Abstract
A novel cap-independent translation has been performed where the ribosome entry is regulated by the 5′-noncoding region (NCR) of L-A virus mRNA. Despite L-A virus mRNA containing neither cap structure nor a poly(A) tail, the reconstructed mRNA encoding the 5′ NCR of L-A virus mRNA and a reporter gene (luciferase) was translated, in yeast lysate, 60 times more efficiently than control mRNA. The 5′ NCR from L-A virus was effective in regulating the recruitment of ribosome in vitro. A possible mechanism in Saccharomyces cerevisiae is also suggested, whereby the ribosome entry is regulated by the 5′ NCR of L-A virus mRNA.
- Subjects
MESSENGER RNA; VIRUSES; GENETIC vectors; LEAVENING agents; SACCHAROMYCES cerevisiae; RIBOSOMES
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 1, p59
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005699202240