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- Title
Iron and zinc binding activity of Escherichia coli topoisomerase I homolog YrdD.
- Authors
Cheng, Zishuo; Tan, Guoqiang; Wang, Wu; Su, Xiaolu; Landry, Aaron; Lu, Jianxin; Ding, Huangen
- Abstract
YrdD, a homolog of the C-terminal zinc-binding region of Escherichia coli topoisomerase I, is highly conserved among proteobacteria and enterobacteria. However, the function of YrdD remains elusive. Here we report that YrdD purified from E. coli cells grown in LB media contains both zinc and iron. Supplement of exogenous zinc in the medium abolishes the iron binding of YrdD in E. coli cells, indicating that iron and zinc may compete for the same metal binding sites in the protein. While the zinc-bound YrdD is able to bind single-stranded (ss) DNA and protect ssDNA from the DNase I digestion in vitro, the iron-bound YrdD has very little or no binding activity for ssDNA, suggesting that the zinc-bound YrdD may have an important role in DNA repair by interacting with ssDNA in cells.
- Subjects
ZINC; ESCHERICHIA coli; DNA topoisomerase I; PROTEOBACTERIA; ENTEROBACTERIACEAE; SINGLE-stranded DNA
- Publication
BioMetals, 2014, Vol 27, Issue 2, p229
- ISSN
0966-0844
- Publication type
Article
- DOI
10.1007/s10534-013-9698-z