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- Title
Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control.
- Authors
Xing-Long Xiao; Ya-Qing He; Yi-Gang Yu; Hong Yang; Gu Chen; Hui-Fang Li; Jing-Wei Zhang; Dong-Mei Liu; Xiao-Feng Li; Xiao-Quan Yang; Hui Wu
- Abstract
The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.
- Subjects
CHINA; ENTEROVIRUSES; ENTEROVIRUS diseases; COXSACKIEVIRUSES; GENE amplification
- Publication
Archives of Virology, 2009, Vol 154, Issue 1, p121
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-008-0266-8