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- Title
Specific detection of avian pneumovirus (APV) US isolates by RT-PCR.
- Authors
Shin, H. J.; Rajashekara, G.; Jirjis, F. F.; Shaw, D. P.; Goyal, S. M.; Halvorson, D. A.; Nagaraja, K. V.
- Abstract
Summary. This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV). Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as low as 8 × 10-5 TCID50 (0.0323 μg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from turkeys in Minnesota.
- Subjects
VIRUS diseases in poultry; NUCLEIC acids; NUCLEOTIDE sequence; TURKEYS; NUCLEOTIDE analysis; REVERSE transcriptase; POLYMERASE chain reaction; DISEASES
- Publication
Archives of Virology, 2000, Vol 145, Issue 6, p1239
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s007050070123