We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes.
- Authors
Chitimia, Lidia; Lin, Rui-Qing; Cosoroaba, Iustin; Wu, Xiang-Yun; Song, Hui-Qun; Yuan, Zi-Guo; Zhu, Xing-Quan
- Abstract
In the present study, samples representing three hard tick species and one soft tick species, namely Dermacentor marginatus, Haemaphysalis punctata, Ixodes ricinus and Argas persicus from southwestern Romania, and one hard tick, Haemaphysalis longicornis, from China were characterized genetically by a portion of mitochondrial cytochrome c oxidase subunit 1 gene (p cox1) and a portion of nicotinamide adenine dinucleotide dehydrogenase subunit 5 gene (p nad5). The p cox1 and p nad5 were amplified separately from individual ticks by PCR, sequenced and analyzed. The length of p cox1 and p nad5 sequences of all samples was 732 and 519 bp, respectively. The intra-specific sequence variation in De. marginatus was 0.1-1.0% for p cox1 and 0.2-1.2% for p nad5 , whereas in Ha. punctata it was 0.4-1.9% for p cox1 and 0.4-1.0% for p nad5 . For the tick species examined in the present study, sequence comparison revealed that the inter-specific sequence differences were higher: 15.9-27.6% for p cox1 and 20.3-42.4% for p nad5. This suggests that the cox1 and nad5 sequences could provide useful genetic markers for the specific identification and genetic characterization of ticks in Romania and elsewhere.
- Subjects
ROMANIA; CHINA; TICKS; ANIMAL genetics; MITOCHONDRIAL DNA; CYTOCHROME c; PHYLOGENY; ENTOMOLOGY
- Publication
Experimental & Applied Acarology, 2010, Vol 52, Issue 3, p305
- ISSN
0168-8162
- Publication type
Article
- DOI
10.1007/s10493-010-9365-9