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- Title
速生桉叶水溶提取物对 HepG2 细胞增殖及凋亡的影响.
- Authors
周金爱; 余军; 黄东萍; 吴睿; 刘欣; 臧宁
- Abstract
Objective: To investigate the effects of the leaf of Fast-growing eucylyptus extract on the proliferation and apoptosis of human hepatoma HepG2 cells. Methods: MTT assay was used to measure the leaf of Fast-growing eucylyptus extract on HepG2 cells activity. The cell apoptosis of HepG2 cells were determined by flow cytometry Annexin V-FITC/PI. Results: As the results of MTT showed, HepG2 cells were cultured for 24 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were (47.32± 1.11) %and (15.76 ± 3.50) %, respectively. When HepG2 cells were cultured for 48 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were respectively (44.13± 10.93)%and (25.93± 8.37) %. When HepG2 cells were cultured for 72 h, the inhibition rate of HepG2 cells diluted 5 and 10 times was (59.47± 6.90)%and (41.02± 4.27) %. The flow cytometry showed that after incubated with the leaf of fast-growing eucylyptus extract for diluted 30 times, the early of apoptosis were 30.03%. The ratios of apoptotic increased with concentration diluted times decreased, and it was in a time-dependent manner. Moreover, the ratios of apoptotic increased with the time prolonged of the leaf of fast-growing eucylyptus extract, and it was in a dose-dependent manner. Conclusion: The leaf of fast-growing eucylyptus extract can inhibit proliferation and induced apoptosis on HepG2 Cells in a time-and dose-dependent manner.
- Subjects
FLOW cytometry; CELL culture
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 24, p4623
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.24.004