We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Mononuclear Cell Antigen Expression after Contact with One-Day Disposable Contact Lens Materials.
- Authors
P. Versura; S. Iannelli; S. Stefoni; R. Caramazza
- Abstract
AbstractPurpose: To analyse the effects of 1-day disposable contact lens (CL) material on mononuclear cell activation antigens and adhesion molecules, in an in vitro model set up in order to simulate the first 2 h of wear. Methods: Etafilcon A and Hilafilcon A materials were analysed. Mononuclear cells, collected from peripheral blood samples of normal human donors, were placed in contact with the materials, both new and coated with artificial tear solution. As a control, Thermanox coverslips and a group with no material were included. Immunofluorescence reactions were carried out on cells, to study the distribution of activation (HLA-DR, CD25, CD69) and adhesion (CD11a, CD18, CD54) molecules; the flow cytometry technique was used to analyse cells. Results: Statistically significant results were demonstrated for the CD69 and all the 3 adhesion molecules. Artificial tear coating enhanced the mononuclear cell response. Conclusions: The daily mononuclear cell activation can lead, with time, to production of inflammatory mediators that may be responsible for discomfort or, at worst, intolerance and definitely to CL wear remittance.Copyright © 2003 S. Karger AG, Basel
- Subjects
CONTACT lenses; ANTIGENS; ADHESION; MOLECULES; CELLS
- Publication
Ophthalmologica, 2003, Vol 217, Issue 3, p225
- ISSN
0030-3755
- Publication type
Article
- DOI
10.1159/000068970