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- Title
Denaturing gradient gel electrophoresis as a tool for the identification of commercially important Crassostrea oysters in China.
- Authors
YU, Hong; LI, Qi
- Abstract
The article reports on a study of the use of denaturing gradient gel electrophoresis (DGGE) as a tool for identifying commercially important Crassostrea oysters in China. It used DGGE, an electrophoresis method, in examining 150 oysters which were stored at 80 degree Celsius until DNA preparation. It extracted genomic DNA from adductor muscle through proteinase K digestion, phenol-chloroform extraction and DNA precipitation. It showed that polymerase chain reaction (PCR) products from different oyster species including Crassostrea gigas and Crassostrea plicatula have the same degree of mobility but did not manifest any divergence. It also founds out that DGGE analysis of the mtDNA segment could be used for identifying and detecting various Crassostrea oysters.
- Subjects
CHINA; DENATURING gradient gel electrophoresis; CRASSOSTREA; OYSTERS; POLYMERASE chain reaction; ELECTROPHORESIS; PROTEINASES; DNA; BIOLOGICAL divergence
- Publication
Fisheries Science, 2008, Vol 74, Issue 6, p1354
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1111/j.1444-2906.2008.01667.x