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- Title
Design and Application of a Novel High-throughput Screening Technique for 1-Deoxynojirimycin.
- Authors
Jiang, Peixia; Mu, Shanshan; Li, Heng; Li, Youhai; Feng, Congmin; Jin, Jian-Ming; Tang, Shuang-Yan
- Abstract
High-throughput screening techniques for small molecules can find intensive applications in the studies of biosynthesis of these molecules. A sensitive, rapid and cost-effective technique that allows high-throughput screening of endogenous production of the natural iminosugar 1-deoxynojirimycin (1-DNJ), an α-glucosidase inhibitor relevant to the pharmaceutical industry, was developed in this study, based on the inhibitory effects of 1-DNJ on the activity of the β-glycosidase LacS from Sulfolobus solfataricus. This technique has been demonstrated effective in engineering both the key enzyme and the expression levels of enzymes in the 1-DNJ biosynthetic pathway from Bacillus atrophaeus cloned in E. coli. Higher biosynthetic efficiency was achieved using directed evolution strategies.
- Subjects
GLUCOSIDASE inhibitors; GAUCHER'S disease; AMINOTRANSFERASES; OXIDOREDUCTASES; SULFOLOBUS solfataricus
- Publication
Scientific Reports, 2015, p8563
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep08563