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- Title
Sex difference in immunostaining of RET in the adult mouse kidney.
- Authors
Chan, S Y; Lee, D C W
- Abstract
The c-ret proto-oncogene encodes a receptor tyrosine kinase which is important for the development of the kidney and the enteric nervous system. During nephrogenesis, c-ret is expressed in the ureteric bud epithelium and later in its derivative, the collecting duct. This takes place during 11–17.5 days post-coitum (d.p.c.) in the mouse and our immunohistochemical study showed that the RET protein co-localized with the transcript. At 18.5 d.p.c. the kidney is fully differentiated. At 18.5 d.p.c., 1 week and 10 weeks old, RET was found in the proximal convoluted tubules, which is formed from the condensed mesenchyme. This suggests that c-ret may also play a role in kidney function. For the 10 weeks old kidney, RET immunostaining in male was concentrated on the basolateral side while female had a stronger staining in the whole cell. Furthermore, cytoplasmic staining was observed in male whereas both cytoplasmic and nuclear staining was found in female. c-ret transcript was detected by RT–PCR, and in situ hybridization showed its expression throughout the kidney. The reason for the sex-specific staining and the role of RET in kidney function remain to be determined.
- Subjects
PROTO-oncogenes; PROTEIN-tyrosine kinases; KIDNEYS; MICE
- Publication
Oncogene, 1998, Vol 17, Issue 5, p661
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1201970