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- Title
Diverse Applications of Affinity Labels.
- Authors
Hartman, Fred C.
- Abstract
The article presents information on diverse applications of affinity labels. Affinity labeling entails the use of reactive analogs of natural substrates (ligands) to label substrate (ligand) binding sites. Other criteria important in establishing active-site modification by affinity labels are, substrate protection, stoichiometric incorporation and necessity of the native three-dimensional structure for reaction to occur. Recent variations on the traditional approaches to affinity labeling are the use of suicide reagents and photoaffinity labels. The greatest challenge of affinity labeling appears to be in drug design, since the mode of action of many naturally occurring antibiotics and synthetic drugs is via the selective, irreversible inactivation of enzymes.
- Subjects
AFFINITY labeling; PROTEIN affinity labeling; PROTEIN binding; PROTEIN analysis; PHOTOAFFINITY labeling; MOLECULAR probes; LIGANDS (Biochemistry); BINDING sites; CELL receptors
- Publication
BioScience, 1978, Vol 28, Issue 4, p270
- ISSN
0006-3568
- Publication type
Article
- DOI
10.2307/1307279