We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
In vivo Investigation of Glutamate-Glutamine Metabolism in Hyperammonemic Monkey Brain Using <sup>13</sup>C-Magnetic Resonance Spectroscopy.
- Authors
Tsukada, Y.; Kanamatsu, T.; Watanabe, H.; Okamoto, K.
- Abstract
To investigate the metabolism of glutamate and glutamine in living monkey brain, a system of in vivo 13C magnetic resonance spectroscopy (MRS) using 1H-decoupled 13C spectroscopy combined with monitoring temperature changes in the brain by MR phase mapping was developed. Serial 13C-NMR spectra of the amino acids glutamate and glutamine were acquired non-invasively over 4 h from anesthetized monkey brain after the intravenous administration of [1-13C]glucose (0.5-1.0 g/kg). In the acute hyperammonemic state induced by the administration of ammonium acetate (77 mg/kg bolus), it was observed that 13C incorporation into glutamine-4 was clearly accelerated, without changes of 13C incorporation into glutamate-4. During hyperammonemia, it was shown directly by [2-13C]glucose administration that the anaplerotic pathway for the TCA cycle was also augmented, contributing to the formation of glutamine in the astroglia.
- Publication
Developmental Neuroscience, 1998, Vol 20, Issue 4/5, p427
- ISSN
0378-5866
- Publication type
Article
- DOI
10.1159/000017340