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- Title
The red microalga Flintiella sanguinaria as a new exopolysaccharide producer.
- Authors
Gaignard, Clément; Macao, Vanessa; Gardarin, Christine; Rihouey, Christophe; Picton, Luc; Michaud, Philippe; Laroche, Céline
- Abstract
Flintiella sanguinaria is a red unicellular microalgae belonging to Porphyridiophyceae with a high potential as an exopolysaccharide producer. A new culture medium was developed by a stoichiometry approach based on elemental analysis of microalgae, and culture conditions were improved after measurements of photosynthetic activity as a function of irradiance, temperature and pH. These optimal conditions were thus validated in a photobioreactor for the production of biomass and exopolysaccharide (EPS). After extraction and purification of the EPS, it was characterised by HPAEC-PAD, HPLC and FT-IR as a methylated and acetylated galactoxylan including a significant amount of rhamnose and glucuronic acid in its structure. Macromolecular conformation in dilute solution of native, deproteinised and desubstituted EPS from F. sanguinaria revealed that this polysaccharide had a strong associative behaviour in which hydrophobic interactions or hydrogen bonding but also proteins were implicated.
- Publication
Journal of Applied Phycology, 2018, Vol 30, Issue 5, p2803
- ISSN
0921-8971
- Publication type
Article
- DOI
10.1007/s10811-018-1389-2