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- Title
Paenimacrolidin, a novel macrolide antibiotic from Paenibacillus sp. F6-B70 active against methicillin-resistant Staphylococcus aureus.
- Authors
Wu, Xue-Chang; Qian, Chao-Dong; Fang, Hai-Huan; Wen, Yan-Ping; Zhou, Jian-Ying; Zhan, Zha-Jun; Ding, Rui; Li, Ou; Gao, Haichun
- Abstract
Summary Paenibacillus sp. F6-B70 was selected from several dozens of isolates with activity against methicillin-resistant Staphylococcus aureus using a 16S rDNA-based screening method. F6-B70 contained polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) clusters in its genome revealed by PCR amplification of conserved adenylation and ketosynthase (KS) domains. Phylogenetic data suggested that the strain hosts trans-AT PKSs and their product may be a branched molecule. An antibiotic was subsequently isolated from the methanol extract of F6-B70 cells. The molecular formula of the antibiotic was deduced to be C33H50NaO6 ([M + Na]+, m/z 565.3505) by analysis of electrospray ionization mass spectral data. Elucidation of the structure by nuclear magnetic resonance and infrared spectroscopy revealed that the active compound, paenimacrolidin (PAM), was a novel 22-membered macrolide with side-chains. The new antibiotic, mainly as a bacteriostatic agent, inhibits a couple of multidrug-resistant Staphylococcus sp. strains. The antibiotic capacity of PAM was compromised by its instability, which can be overcome significantly with addition of an anti-oxidant. To our knowledge, this is the first report of the isolation of an active macrolide from paenibacilli, which may be a promising source of novel antibiotics.
- Subjects
METHICILLIN-resistant staphylococcus aureus; ANTIBACTERIAL agents; ANTI-infective agents; POLYKETIDE synthases; PEPTIDE synthesis; GENOMES
- Publication
Microbial Biotechnology, 2011, Vol 4, Issue 4, p491
- ISSN
1751-7907
- Publication type
Article
- DOI
10.1111/j.1751-7915.2010.00201.x