We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
d-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants
- Authors
Wienstroer, Judith; Engqvist, Martin K.M.; Kunz, Hans-Henning; Flügge, Ulf-Ingo; Maurino, Veronica G.
- Abstract
Abstract: d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5–10mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase (d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.
- Subjects
LACTATE dehydrogenase; TRANSGENIC plants; ARABIDOPSIS thaliana; GERMINATION; PLANT development; GENES
- Publication
FEBS Letters, 2012, Vol 586, Issue 1, p36
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.11.020