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- Title
Characteristic morphology of intracellular microcolonies of Legionella oakridgensis OR-10.
- Authors
Takekawa, Yuta; Saito, Mitsumasa; Wang, Changle; Qin, Tian; Ogawa, Midori; Kanemaru, Takaaki; Yoshida, Shin-ichi
- Abstract
Legionella oakridgensis occasionally causes pneumonia in humans. We report here the characteristic morphology of intracellular microcolonies of L. oakridgensis OR-10 in infected epithelial cells. By light microscopy after Gimenez staining, the bacteria showed serpentine-like chain, disk-like conglomerate, and granular forms when they grew intracellularly in Vero cells, HeLa cells, and A549 cells. In a time-lapse study, we observed the progressive change from a serpentine-like chain form to a conglomerate form in Vero cells. Transmission electron microscopy showed that L. oakridgensis OR-10 proliferated both inside membrane structures and in the cytoplasm. Such highly serpentine chain growth has not been reported in any intracellular bacteria. Furthermore, these results imply that L. oakridgensis OR-10 may be proliferating inside the endoplasmic reticulum.
- Subjects
LEGIONELLA; CELL morphology; PNEUMONIA; BACTERIAL colonies; CYTOPLASM; TRANSMISSION electron microscopy
- Publication
Canadian Journal of Microbiology, 2012, Vol 58, Issue 2, p179
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w11-126