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- Title
New Culture Conditions for Clostridium histolyticum leading to Production of Collagenase of High Specific Activity.
- Authors
Takahashi, S.; Seifter, S.
- Abstract
S ummary: New culture conditions are described for the growth of Clostridium histolyticum allowing for the production of collagenase (E.C.3.4.4.19) in a highly purified form, free of general protease activity. The conditions include the use of a mixture of sodium bisulphite-oxidized sodium thioglycollate to provide the reducing environment within an otherwise standard culture medium. Also, new modifications in the preparation of collagenase A are reported.
- Publication
Journal of Applied Microbiology, 1972, Vol 35, Issue 4, p647
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/j.1365-2672.1972.tb03746.x